Three-dimensional differential interference contrast microscopy using synthetic aperture imaging

J Biomed Opt. 2012 Feb;17(2):026003. doi: 10.1117/1.JBO.17.2.026003.

Abstract

We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Imaging, Three-Dimensional / instrumentation*
  • Lenses*
  • Microscopy, Phase-Contrast / instrumentation*
  • Reproducibility of Results
  • Sensitivity and Specificity