High-performance liquid chromatography for the separation of angiotensin and its metabolites in human plasma and sweat

J Chromatogr Sci. 1990 Oct;28(10):524-8. doi: 10.1093/chromsci/28.10.524.

Abstract

A reversed-phase high-performance liquid chromatography (HPLC) method with gradient elution for the separation of angiotensin peptides is described. The highly reproducible method allows the base-line separation of angiotensin peptides with UV detection at 225 nm. This chromatographic methodology in combination with radioimmunoassay (RIA) is used for the characterization of angiotensin peptides in human plasma and sweat.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetonitriles
  • Angiotensin II / analysis
  • Angiotensin II / blood*
  • Angiotensin II / metabolism
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Methanol
  • Radioimmunoassay
  • Sweat / chemistry*

Substances

  • Acetonitriles
  • Angiotensin II
  • Methanol
  • acetonitrile