Abstract
Developing an effective vaccine against HIV infection remains an urgent goal. We used a DNA prime/fowlpox virus boost regimen to immunize Chinese rhesus macaques. The animals were challenged intramuscularly with pathogenic molecularly cloned SHIV-KB9. Immunogenicity and protective efficacy of vaccines were investigated by measuring IFN-γ levels, monitoring HIV-specific binding antibodies, examining viral load, and analyzing CD4/CD8 ratio. Results show that, upon challenge, the vaccine group can induce a strong immune response in the body, represented by increased expression of IFN-γ, slow and steady elevated antibody production, reduced peak value of acute viral load, and increase in the average CD4/CD8 ratio. The current research suggests that rapid reaction speed, appropriate response strength, and long-lasting immune response time may be key protection factors for AIDS vaccine. The present study contributes significantly to AIDS vaccine and preclinical research.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS Vaccines / administration & dosage*
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AIDS Vaccines / genetics
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Animals
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CD4-CD8 Ratio
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China
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DNA, Viral / genetics
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DNA, Viral / immunology*
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Female
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Fowlpox virus / genetics
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Fowlpox virus / immunology*
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HIV / genetics
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HIV / immunology
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HIV Antibodies / biosynthesis
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HIV Antibodies / immunology
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HIV Core Protein p24 / genetics
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HIV Core Protein p24 / immunology*
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HIV Infections / immunology
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HIV Infections / prevention & control
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HIV Infections / virology
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Humans
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Immunization, Secondary
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Injections, Intramuscular
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Interferon-gamma / biosynthesis
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Interferon-gamma / immunology
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Macaca mulatta
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Male
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Simian Acquired Immunodeficiency Syndrome / immunology
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Simian Acquired Immunodeficiency Syndrome / prevention & control*
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Simian Acquired Immunodeficiency Syndrome / virology
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Simian Immunodeficiency Virus / drug effects*
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Simian Immunodeficiency Virus / physiology
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Vaccination*
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Vaccines, DNA
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Viral Load / drug effects
Substances
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AIDS Vaccines
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DNA, Viral
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HIV Antibodies
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HIV Core Protein p24
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Vaccines, DNA
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Interferon-gamma