Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate

Keiichi Ohtsuka; Shinobu Sato; Yusuke Sato; Kojiro Sota; Shinsuke Ohzawa; Tomoki Matsuda; Kiwamu Takemoto; Nobutoki Takamune; Bernard Juskowiak; Takeharu Nagai; Shigeori Takenaka

doi:10.1039/c2cc30536d

Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate

Chem Commun (Camb). 2012 May 16;48(39):4740-2. doi: 10.1039/c2cc30536d. Epub 2012 Apr 5.

Authors

Keiichi Ohtsuka¹, Shinobu Sato, Yusuke Sato, Kojiro Sota, Shinsuke Ohzawa, Tomoki Matsuda, Kiwamu Takemoto, Nobutoki Takamune, Bernard Juskowiak, Takeharu Nagai, Shigeori Takenaka

Affiliation

¹ Department of Applied Chemistry, Graduate School of Engineering, Kyushu Institute of Technology, Fukuoka 804-8550, Japan.

PMID: 22475983
DOI: 10.1039/c2cc30536d

Abstract

When a biotinylated FRET probe based on a peptide-thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K(+) concentration changes in the cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Fluorescence Resonance Energy Transfer
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Ions
Peptides / chemistry*
Potassium / analysis
Potassium / chemistry*
Streptavidin / chemistry

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Ions
Peptides
thrombin aptamer
Streptavidin
Potassium