Abstract
Despite the availability of a Mycobacterium bovis bacille Calmette Guérin (BCG) vaccine, tuberculosis (TB) remains a global public health problem. In this study, we introduced the c-di-GMP phosphodiesterase gene Rv1357c, implicated in regulating mycobacterial replication within macrophages, into BCG Pasteur, and tested the resulting strain for its capacity to serve as a vaccine against TB in a murine model. Modified BCG was more phagocytosed than its parental strain, but halted bacterial replication, and protected against M. tuberculosis challenge similarly to unmodified BCG.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acyltransferases / genetics
-
Acyltransferases / immunology*
-
Animals
-
BCG Vaccine / immunology*
-
Cell Line
-
Disease Models, Animal
-
Macrophages / immunology
-
Macrophages / microbiology
-
Mice
-
Mice, Inbred BALB C
-
Mycobacterium tuberculosis / genetics
-
Mycobacterium tuberculosis / growth & development
-
Mycobacterium tuberculosis / immunology*
-
Phagocytosis
-
Time Factors
-
Tuberculosis, Pulmonary / immunology
-
Tuberculosis, Pulmonary / microbiology
-
Tuberculosis, Pulmonary / prevention & control*
-
Vaccination*
-
Vaccines, Synthetic / immunology
Substances
-
BCG Vaccine
-
Vaccines, Synthetic
-
Acyltransferases
-
Rv1347c, Mycobacterium tuberculosis