Immunogenicity and efficacy of an anthrax/plague DNA fusion vaccine in a mouse model

FEMS Immunol Med Microbiol. 2012 Aug;65(3):505-9. doi: 10.1111/j.1574-695X.2012.00974.x. Epub 2012 May 21.

Abstract

The efficacy of multi-agent DNA vaccines consisting of a truncated gene encoding Bacillus anthracis lethal factor (LFn) fused to either Yersinia pestis V antigen (V) or Y . pestis F1 was evaluated. A/J mice were immunized by gene gun and developed predominantly IgG1 responses that were fully protective against a lethal aerosolized B. anthracis spore challenge but required the presence of an additional DNA vaccine expressing anthrax protective antigen to boost survival against aerosolized Y. pestis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anthrax / immunology
  • Anthrax / mortality
  • Anthrax / prevention & control*
  • Anthrax Vaccines / administration & dosage
  • Anthrax Vaccines / immunology*
  • Antibodies, Bacterial / blood
  • Antibodies, Bacterial / immunology
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology
  • Bacillus anthracis / immunology
  • Bacillus anthracis / pathogenicity
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology
  • Bacterial Toxins / genetics
  • Bacterial Toxins / immunology
  • Biolistics
  • Immunization
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Mice
  • Plague / immunology
  • Plague / mortality
  • Plague / prevention & control*
  • Plague Vaccine / administration & dosage
  • Plague Vaccine / immunology*
  • Pore Forming Cytotoxic Proteins / genetics
  • Pore Forming Cytotoxic Proteins / immunology
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Spores, Bacterial / immunology
  • Spores, Bacterial / pathogenicity
  • Survival Rate
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology
  • Yersinia pestis / immunology

Substances

  • Anthrax Vaccines
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Bacterial Toxins
  • Immunoglobulin G
  • LcrV protein, Yersinia
  • Plague Vaccine
  • Pore Forming Cytotoxic Proteins
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic
  • anthrax toxin
  • caf1 protein, Yersinia pestis