Dynamics in multi-domain protein recognition of RNA

Cameron D Mackereth; Michael Sattler

doi:10.1016/j.sbi.2012.03.013

Dynamics in multi-domain protein recognition of RNA

Curr Opin Struct Biol. 2012 Jun;22(3):287-96. doi: 10.1016/j.sbi.2012.03.013. Epub 2012 Apr 18.

Authors

Cameron D Mackereth¹, Michael Sattler

Affiliation

¹ Institut Européen de Chimie et Biologie, Inserm U869 and Univ. Bordeaux, 2 rue Robert Escarpit, 33607 Pessac, France. [email protected]

PMID: 22516180
DOI: 10.1016/j.sbi.2012.03.013

Abstract

Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combination and cooperation of these interactions. Recent structures, together with biochemical and biophysical studies have revealed novel principles of RNA recognition by multi-domain proteins. These examples highlight an important role for dynamics in RNA recognition, with mechanisms including fly-casting and conformational selection, and advocate the use of solution techniques for their analysis.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Models, Molecular*
Nucleic Acid Conformation
Protein Binding
Protein Conformation
Protein Interaction Domains and Motifs
RNA / chemistry*
RNA / metabolism
RNA-Binding Proteins / chemistry*
RNA-Binding Proteins / metabolism

Substances

RNA-Binding Proteins
RNA