Abstract
We present a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems. This WormToolbox is available through the open-source CellProfiler project and enables objective scoring of whole-worm high-throughput image-based assays of C. elegans for the study of diverse biological pathways that are relevant to human disease.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Algorithms
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Animals
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Caenorhabditis elegans / cytology*
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High-Throughput Screening Assays* / instrumentation
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High-Throughput Screening Assays* / methods
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Image Processing, Computer-Assisted* / instrumentation
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Image Processing, Computer-Assisted* / methods
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods*
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Pattern Recognition, Automated / methods*
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Phenotype
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Software