Abstract
Chromatin immunoprecipitation (ChIP) followed by microarray-based (ChIP-Chip) or next-generation sequencing-based (ChIP-Seq) analysis has been established as a powerful and widely used method to investigate DNA-protein interactions relative to a genomic location in vivo. Here, we present a ChIP-Chip protocol, which utilizes an alternative, easier amplification protocol and when using high-quality ChIP-grade antibodies, will generate enough material for hybridization or sequencing with negligible enrichment bias due to amplification.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatin / genetics
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Chromatin / metabolism
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Chromatin Immunoprecipitation / methods*
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Embryonic Stem Cells / metabolism*
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Gene Regulatory Networks / genetics
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Gene Regulatory Networks / physiology*
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Humans
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Nanog Homeobox Protein
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Octamer Transcription Factor-3 / genetics
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Octamer Transcription Factor-3 / metabolism
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Oligonucleotide Array Sequence Analysis
Substances
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Chromatin
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Homeodomain Proteins
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NANOG protein, human
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Nanog Homeobox Protein
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Octamer Transcription Factor-3
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POU5F1 protein, human