Electrospun TiO2 nanofiber-based cell capture assay for detecting circulating tumor cells from colorectal and gastric cancer patients

Adv Mater. 2012 May 22;24(20):2756-60. doi: 10.1002/adma.201200155. Epub 2012 Apr 23.

Abstract

A nanostructured platform that combines electrospun TiO(2) nanofibers (TiNFs)-deposited substrate and cell-capture agent realizes significant capture of circulating tumor cells (CTCs). The enhanced local topographic interactions between the horizontally packed TiNFs deposited substrates and extracellular matrix scaffolds, in addition to anti-EpCAM/EpCAM biological recognition, contributes to the significantly enhanced capture efficiency compared to flat surfaces.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / metabolism
  • Cell Adhesion Molecules / metabolism
  • Cell Line, Tumor
  • Colorectal Neoplasms / blood*
  • Colorectal Neoplasms / pathology
  • Epithelial Cell Adhesion Molecule
  • HCT116 Cells
  • HeLa Cells
  • Humans
  • Immunohistochemistry
  • K562 Cells
  • Nanofibers / chemistry*
  • Neoplastic Cells, Circulating* / metabolism
  • Povidone / chemistry
  • Stomach Neoplasms / blood*
  • Stomach Neoplasms / pathology
  • Titanium / chemistry*

Substances

  • Antigens, Neoplasm
  • Cell Adhesion Molecules
  • Epithelial Cell Adhesion Molecule
  • titanium dioxide
  • Titanium
  • Povidone