Gene expression profiling in acute Stanford type B aortic dissection

Vasc Endovascular Surg. 2012 May;46(4):300-9. doi: 10.1177/1538574412443315. Epub 2012 Apr 25.

Abstract

Objective: To compare the gene expression profiles of the aorta specimens between patients with Stanford type B aortic dissection (AD) and controls.

Materials and methods: Samples of descending aorta were collected from patients with type B AD (n = 12) and from multiorgan donors as controls (n = 12). Phalanx whole genome microarray was used to analyze differential gene expression.

Results: Of the 6375 probes validated, 623 genes were found to be differentially expressed between patients with type B AD and controls (fold change ≥2). Gene ontology analysis identified significantly enriched gene groups pertaining to cell-cell adhesion, extracellular matrix, cell-matrix adhesion, cytoskeleton, immune and inflammatory response, and apoptosis.

Conclusion: Genes encoding components related to integrity and strength of the aortic wall were downregulated, whereas those related to inflammatory response were upregulated in type B AD. The altered patterns of gene expression indicate preexisting structural defects that are probably a consequence of insufficient remodeling of the aortic wall.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aortic Aneurysm / genetics*
  • Aortic Aneurysm / metabolism
  • Aortic Aneurysm / pathology
  • Aortic Aneurysm / surgery
  • Aortic Dissection / genetics*
  • Aortic Dissection / metabolism
  • Aortic Dissection / pathology
  • Aortic Dissection / surgery
  • Case-Control Studies
  • China
  • Cluster Analysis
  • Female
  • Gene Expression Profiling* / methods
  • Gene Expression Regulation
  • Gene Regulatory Networks
  • Humans
  • Male
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • Real-Time Polymerase Chain Reaction