Abstract
To facilitate sequence-specific detection of DNA amplified in a diagnostic reverse transcription (RT)-PCR for Lassa virus, we developed an array featuring 47 oligonucleotide probes for post-PCR hybridization of the amplicons. The array procedure may be performed with low-tech equipment and does not take longer than agarose gel detection.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Clinical Laboratory Techniques / methods
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Electrophoresis, Agar Gel
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Humans
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Lassa Fever / diagnosis*
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Lassa Fever / virology
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Lassa virus / genetics
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Lassa virus / isolation & purification*
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Microarray Analysis / methods*
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Oligonucleotide Array Sequence Analysis / methods*
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Oligonucleotide Probes / genetics
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Time Factors
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Virology / methods*