Formation of one-way-structured cultured neuronal networks in microfluidic devices combining with micropatterning techniques

Yuzo Takayama; Naoki Kotake; Tatsuya Haga; Takafumi Suzuki; Kunihiko Mabuchi

doi:10.1016/j.jbiosc.2012.02.011

Formation of one-way-structured cultured neuronal networks in microfluidic devices combining with micropatterning techniques

J Biosci Bioeng. 2012 Jul;114(1):92-5. doi: 10.1016/j.jbiosc.2012.02.011. Epub 2012 Apr 24.

Authors

Yuzo Takayama¹, Naoki Kotake, Tatsuya Haga, Takafumi Suzuki, Kunihiko Mabuchi

Affiliation

¹ Graduate School of Information Science and Technology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. [email protected]

PMID: 22538184
DOI: 10.1016/j.jbiosc.2012.02.011

Abstract

We present a simple method to regulate the direction of axon development in cultured neurons using microfabrication and microfluidics techniques. We fabricate a PDMS-based device and place it onto a chemically micropatterned glass substrate. We confirm that cultured neurons extend neurites along the medium flow direction and the micropatterned regions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Axons / physiology
Embryo, Mammalian
Glass
Microfluidic Analytical Techniques / instrumentation*
Nerve Net / cytology
Nerve Net / embryology*
Neurites / physiology
Neurons / cytology*
Rats
Rats, Wistar