[Microbiological diagnosis of mycoplasma infections]

María Antonia Meseguer-Peinado; Beatriz Acosta-Boga; Lurdes Matas-Andreu; Gemma Codina-Grau

doi:10.1016/j.eimc.2011.10.020

[Microbiological diagnosis of mycoplasma infections]

Enferm Infecc Microbiol Clin. 2012 Oct;30(8):500-4. doi: 10.1016/j.eimc.2011.10.020. Epub 2012 Apr 27.

[Article in Spanish]

Authors

María Antonia Meseguer-Peinado¹, Beatriz Acosta-Boga, Lurdes Matas-Andreu, Gemma Codina-Grau

Affiliation

¹ Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Madrid, España. [email protected]

PMID: 22541339
DOI: 10.1016/j.eimc.2011.10.020

Abstract

The microbiological diagnosis of mycoplasma and ureaplasma infections has always been limited due to the fastidious growth of these microorganisms, as well as the lack of commercially prepared growth media, absence of rapid diagnostic procedures, and the clinical perception that these organisms are less significant in the infectious diseases setting. During the last few years, this situation has substantially improved due to the commercial availability of culture media, the development of rapid serological techniques, and, in particular, to the introduction of nucleic acid amplification assays, commercially available or "in-house" preparations. Despite the lack of proper standardisation and validation of the molecular and serological techniques, methodological advances have led to an increased detection of these microorganisms and, consequently, a greater appreciation of their clinical relevance.

Publication types

English Abstract
Review

MeSH terms

Antibodies, Bacterial / blood
Bacteriological Techniques*
Culture Media
DNA, Bacterial / blood
Female
Female Urogenital Diseases / diagnosis
Female Urogenital Diseases / microbiology
Humans
Male
Male Urogenital Diseases / diagnosis
Male Urogenital Diseases / microbiology
Mass Spectrometry
Mycoplasma / drug effects
Mycoplasma / growth & development
Mycoplasma / isolation & purification
Mycoplasma / pathogenicity
Mycoplasma Infections / diagnosis*
Mycoplasma Infections / microbiology
Pneumonia, Mycoplasma / diagnosis
Pneumonia, Mycoplasma / microbiology
Pregnancy
Pregnancy Complications, Infectious / diagnosis
Pregnancy Complications, Infectious / microbiology
Real-Time Polymerase Chain Reaction
Serologic Tests / methods
Species Specificity
Specimen Handling
Spectrum Analysis, Raman
Ureaplasma / drug effects
Ureaplasma / growth & development
Ureaplasma / isolation & purification
Ureaplasma / pathogenicity
Ureaplasma Infections / diagnosis*
Ureaplasma Infections / microbiology
Virulence

Substances

Antibodies, Bacterial
Culture Media
DNA, Bacterial