Abstract
RUNX family proteins are critical regulators of lineage differentiation during development. The high prevalence of RUNX mutation/epigenetic inactivation in human cancer indicates a causative role for dysfunctional RUNX in carcinogenesis. This is supported by well-documented evidence of functional interaction of RUNX with components of major oncogenic or tumor suppressive signaling pathways such as TGFβ and Wnt. Here, we explore the binding partners of RUNX3 proteins to further define the scope of RUNX3 function. Using a mass spectrometry-based approach, we found that RUNX3 binds to centrosomal protein rootletin. This led us to uncover the presence of RUNX proteins at the centrosome. Our findings suggest a potential function for RUNX3 during mitosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Cell Line, Tumor
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Centrosome / metabolism*
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Chlorocebus aethiops
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Core Binding Factor Alpha 1 Subunit / antagonists & inhibitors
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Core Binding Factor Alpha 1 Subunit / genetics
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Core Binding Factor Alpha 1 Subunit / metabolism
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Core Binding Factor Alpha 2 Subunit / antagonists & inhibitors
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Core Binding Factor Alpha 2 Subunit / genetics
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Core Binding Factor Alpha 2 Subunit / metabolism
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Core Binding Factor Alpha 3 Subunit / antagonists & inhibitors
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Core Binding Factor Alpha 3 Subunit / genetics
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Core Binding Factor Alpha 3 Subunit / metabolism*
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Cytoskeletal Proteins / metabolism
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G2 Phase Cell Cycle Checkpoints / drug effects
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HCT116 Cells
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HeLa Cells
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Humans
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Mass Spectrometry
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Mitosis
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Nocodazole / pharmacology
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Protein Binding
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RNA Interference
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RNA, Small Interfering / metabolism
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Signal Transduction
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Tubulin / metabolism
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Wnt Proteins / metabolism
Substances
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CROCC protein, human
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Core Binding Factor Alpha 1 Subunit
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Core Binding Factor Alpha 2 Subunit
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Core Binding Factor Alpha 3 Subunit
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Cytoskeletal Proteins
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RNA, Small Interfering
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Tubulin
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Wnt Proteins
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Nocodazole