Abstract
A new lupane-triterpene, 3β-cis-feruloyloxy-16β-hydroxylup-20(29)-ene (1) were isolated from the ethyl acetate extract of Panax ginseng seeds along with the known compound, 3β-transferuloyloxy-16β-hydroxylup-20(29)-ene (2). Compound 2 was isolated from this plant for the first time. Their chemical structures were determined by mass spectroscopy and one-dimensional and two-dimensional magnetic resonance spectra. The bioactive effects of these compounds on TNF-α-induced NF-κB transcription were evaluated in transfected HepG2 cells. Effects on the expression of NF-κB target genes were also examined using a reverse transcription-polymerase chain reaction. Both compounds 1 and 2 were inhibited NF-κB activity in HepG2 cells by decreasing the cellular concentrations of inflammatory factors iNOS and COX2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Cell Survival / drug effects
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Cyclooxygenase 2 / metabolism
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Dose-Response Relationship, Drug
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Hep G2 Cells
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Humans
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Magnetic Resonance Spectroscopy
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Molecular Structure
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NF-kappa B / antagonists & inhibitors*
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NF-kappa B / genetics
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Nitric Oxide Synthase Type II / antagonists & inhibitors
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Panax / chemistry*
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Reverse Transcriptase Polymerase Chain Reaction
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Seeds / chemistry
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Spectrometry, Mass, Electrospray Ionization
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Transfection
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Triterpenes / chemistry
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Triterpenes / isolation & purification
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Triterpenes / pharmacology*
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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NF-kappa B
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Triterpenes
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lupane
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NOS2 protein, human
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2