Abstract
A PCR for protein D (hpd#3) was used to differentiate nontypeable Haemophilus influenzae (NTHI) from Haemophilus haemolyticus. While 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 Indigenous Australian children with bronchiectasis had phenotypic NTHI isolates confirmed as H. influenzae, only 39% of oropharyngeal specimens with phenotypic NTHI had H. influenzae. The nasopharynx is therefore the preferred site for NTHI colonization studies, and NTHI is confirmed as an important lower-airway pathogen.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Australia
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Bacterial Proteins / genetics
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Bacteriological Techniques / methods*
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Bronchiectasis / complications*
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Child
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Child, Preschool
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Female
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Haemophilus / classification*
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Haemophilus / genetics
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Haemophilus / growth & development
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Haemophilus / isolation & purification*
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Haemophilus Infections / diagnosis*
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Haemophilus Infections / microbiology*
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Humans
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Infant
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Lipoproteins / genetics
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Male
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Nasopharynx / microbiology
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Oropharynx / microbiology
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Polymerase Chain Reaction / methods*
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Population Groups
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Respiratory System / microbiology
Substances
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Bacterial Proteins
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Lipoproteins