SUMOylation of Blimp-1 is critical for plasma cell differentiation

EMBO Rep. 2012 Jun 29;13(7):631-7. doi: 10.1038/embor.2012.60.

Abstract

Transcriptional repressor B lymphocyte-induced maturation protein-1 (Blimp-1) is a master regulator of plasma cell differentiation. Here we show that Blimp-1 is covalently modified by SUMO1 at lysine 816, a modification mediated by SUMO E3 ligase PIAS1. Mutation of Blimp-1 lysine 816 reduces transcriptional repression--correlating with a reduced interaction with a histone deacetylase, HDAC2--and impairs differentiation of antibody-secreting cells. Thus, the SUMO pathway critically regulates Blimp-1 function during plasma cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation*
  • Cell Line
  • Gene Expression Regulation
  • Histone Deacetylase 2 / metabolism
  • Lysine / metabolism
  • Mice
  • Mice, Knockout
  • Mutation
  • Plasma Cells / cytology*
  • Plasma Cells / metabolism*
  • Positive Regulatory Domain I-Binding Factor 1
  • Protein Inhibitors of Activated STAT / metabolism
  • SUMO-1 Protein / metabolism*
  • Sumoylation*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic

Substances

  • Pias1 protein, mouse
  • Prdm1 protein, mouse
  • Protein Inhibitors of Activated STAT
  • SUMO-1 Protein
  • Transcription Factors
  • Positive Regulatory Domain I-Binding Factor 1
  • Hdac2 protein, mouse
  • Histone Deacetylase 2
  • Lysine