Abstract
Transcriptional repressor B lymphocyte-induced maturation protein-1 (Blimp-1) is a master regulator of plasma cell differentiation. Here we show that Blimp-1 is covalently modified by SUMO1 at lysine 816, a modification mediated by SUMO E3 ligase PIAS1. Mutation of Blimp-1 lysine 816 reduces transcriptional repression--correlating with a reduced interaction with a histone deacetylase, HDAC2--and impairs differentiation of antibody-secreting cells. Thus, the SUMO pathway critically regulates Blimp-1 function during plasma cell differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Differentiation*
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Cell Line
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Gene Expression Regulation
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Histone Deacetylase 2 / metabolism
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Lysine / metabolism
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Mice
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Mice, Knockout
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Mutation
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Plasma Cells / cytology*
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Plasma Cells / metabolism*
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Positive Regulatory Domain I-Binding Factor 1
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Protein Inhibitors of Activated STAT / metabolism
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SUMO-1 Protein / metabolism*
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Sumoylation*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic
Substances
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Pias1 protein, mouse
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Prdm1 protein, mouse
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Protein Inhibitors of Activated STAT
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SUMO-1 Protein
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Transcription Factors
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Positive Regulatory Domain I-Binding Factor 1
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Hdac2 protein, mouse
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Histone Deacetylase 2
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Lysine