Comparison of commercial molecular assays for toxigenic Clostridium difficile detection in stools: BD GeneOhm Cdiff, XPert C. difficile and illumigene C. difficile

Céline Viala; Alban Le Monnier; Naouale Maataoui; Clotilde Rousseau; Anne Collignon; Isabelle Poilane

doi:10.1016/j.mimet.2012.04.017

Comparison of commercial molecular assays for toxigenic Clostridium difficile detection in stools: BD GeneOhm Cdiff, XPert C. difficile and illumigene C. difficile

J Microbiol Methods. 2012 Aug;90(2):83-5. doi: 10.1016/j.mimet.2012.04.017. Epub 2012 Apr 28.

Authors

Céline Viala¹, Alban Le Monnier, Naouale Maataoui, Clotilde Rousseau, Anne Collignon, Isabelle Poilane

Affiliation

¹ Laboratoire de Microbiologie, Hôpital Jean Verdier, Assistance Publique - Hôpitaux de Paris, Bondy, France.

PMID: 22565213
DOI: 10.1016/j.mimet.2012.04.017

Abstract

Three commercial molecular assays were evaluated for toxigenic Clostridium difficile detection in stools. As compared to toxigenic culture, BD GeneOhm Cdiff (BD Diagnostics), XPert C. difficile (Cepheid) and illumigene C. difficile (Meridian Bioscience) demonstrated respectively a sensitivity of 95.5%, 97.8% and 86.7% and a specificity of 97.9%, 97.9% and 100%.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Bacterial Toxins / metabolism*
Bacteriological Techniques / methods*
Clostridioides difficile / genetics
Clostridioides difficile / isolation & purification*
Clostridioides difficile / pathogenicity
Clostridium Infections / diagnosis*
Clostridium Infections / microbiology*
Feces / microbiology*
Humans
Molecular Diagnostic Techniques / methods*
Reagent Kits, Diagnostic
Sensitivity and Specificity

Substances

Bacterial Toxins
Reagent Kits, Diagnostic