YrhB is a highly stable small protein with unique chaperone-like activity in Escherichia coli BL21(DE3)

FEBS Lett. 2012 Apr 5;586(7):1044-8. doi: 10.1016/j.febslet.2012.02.051. Epub 2012 Mar 8.

Abstract

Escherichia coli YrhB (10.6 kDa) from strain BL21(DE3) that is commonly used for protein overexpression is a stable chaperone-like protein and indispensable for supporting the growth of BL21(DE3) at 48 °C but not defined as conventional heat shock protein (HSP). YrhB effectively prevented heat-induced aggregation of ribonucleotide synthetase (PurK). Without ATP, YrhB alone promoted in vitro refolding of uridine phosphorylase (UDP) and protected thermal denaturation of the refolded UDP. As a cis-acting fusion partner, YrhB also significantly reduced inclusion body formation of nine aggregation-prone heterologous proteins in BL21(DE3). Unlike conventional small HSPs, YrhB remained monomer under heat shock condition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Carboxy-Lyases / chemistry
  • Carboxy-Lyases / metabolism
  • DNA-Directed RNA Polymerases / genetics
  • DNA-Directed RNA Polymerases / metabolism
  • Enzyme Stability
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism*
  • Escherichia coli Proteins / chemistry
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism*
  • Gene Expression Regulation, Enzymologic
  • Heat-Shock Response
  • Hot Temperature
  • Inclusion Bodies
  • Kinetics
  • Molecular Chaperones / chemistry
  • Molecular Chaperones / genetics
  • Molecular Chaperones / metabolism*
  • Molecular Weight
  • Mutation
  • Promoter Regions, Genetic
  • Protein Denaturation
  • Protein Refolding
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sigma Factor / genetics
  • Sigma Factor / metabolism
  • Uridine Phosphorylase / chemistry
  • Uridine Phosphorylase / metabolism

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • Molecular Chaperones
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Sigma Factor
  • YrhB protein, E coli
  • Uridine Phosphorylase
  • RNA polymerase sigma 70
  • DNA-Directed RNA Polymerases
  • Carboxy-Lyases
  • purK protein, E coli