Investigation of Borrelia spp. in ticks (Acari: Ixodidae) at the border crossings between China and Russia in Heilongjiang Province, China

Asian Pac J Trop Med. 2012 Jun;5(6):459-64. doi: 10.1016/S1995-7645(12)60078-9.

Abstract

Objective: To investigate the precise species of tick vector and the Borrelia spirochete pathogen at the Heilongjiang Province international border with Russia.

Methods: In this study, ticks were collected from 12 Heilongjiang border crossings (including grasslands, shrublands, forests, and plantantions) to determine the rate and species type of spirochete-infected ticks and the most prevalent spirochete genotypes.

Results: The ticks represented three genera and four species of the Ixodidae family [Ixodes persulcatus, Dermacentor silvarum, Haemaphysalis concinna and Haemaphysalis japonica]. Ixodes persulcatus had the highest amount of Borrelia burgdorferi sensu lato infection of 25.6% and the most common species of Borrelia isolated from Ixodes persulcatus was Borrelia garinii, strain PD91.

Conclusions: Our results suggest that Borrelia garinii PD91-infected Ixodes persulcatus may be the principal cause of Lyme disease in the border crossing areas of Heilongjiang Province.

MeSH terms

  • Animals
  • Arachnid Vectors / microbiology*
  • Borrelia burgdorferi Group / genetics*
  • Borrelia burgdorferi Group / isolation & purification
  • China
  • Emigration and Immigration
  • Ixodes / microbiology*
  • Lyme Disease / microbiology
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA / genetics
  • Russia

Substances

  • RNA