Magnetic capture hybridization of Batrachochytrium dendrobatidis genomic DNA

J Microbiol Methods. 2012 Sep;90(3):156-9. doi: 10.1016/j.mimet.2012.04.021. Epub 2012 May 2.

Abstract

We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream PCR-based genomic applications.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anura / microbiology
  • Biotin / chemistry
  • Chytridiomycota / genetics*
  • DNA Probes / chemistry
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification*
  • Genome, Fungal
  • Immobilized Proteins / chemistry
  • Magnetic Phenomena
  • Microspheres
  • Nucleic Acid Amplification Techniques
  • Nucleic Acid Hybridization
  • Skin / microbiology
  • Streptavidin / chemistry

Substances

  • DNA Probes
  • DNA, Fungal
  • Immobilized Proteins
  • Biotin
  • Streptavidin