Remote efficacy for two different forms of hyaluronate-based adhesion barriers

J Invest Surg. 2012 Jun;25(3):174-80. doi: 10.3109/08941939.2011.615894.

Abstract

Background: Chemically modified sodium hyaluronate and carboxymethylcellulose (HA/CMC) membrane clinically reduces adhesion formation following surgery but was not designed for laparoscopic use. HA/CMC powder of identical chemical composition has been developed to allow for application laparoscopically. We compared the adhesion reduction efficacy of HA/CMC powder and film when applied directly to or remote from sites of surgical trauma. We also investigated the effect of the powder on wound healing.

Materials and methods: Two animal models of adhesion formation were used to evaluate efficacy: a rat peritoneal sidewall defect model and a rabbit cecal abrasion/sidewall defect model. The products were applied directly to the defect or the contralateral sidewall. Adhesions were examined seven days after surgery. In a separate study, the effect of the powder on healing was evaluated at 5, 7, and 28 days using a rat incisional wound strength model.

Results: HA/CMC powder and film, when applied directly to the peritoneal defect, significantly reduced adhesions relative to the untreated control in both models. Remote applications of HA/CMC powder also reduced adhesions. In contrast, remote applications of HA/CMC film had no effect. HA/CMC powder did not significantly alter incisional wound strength at any of the timepoints tested.

Conclusion: In our preclinical models, HA/CMC powder had similar adhesion reduction efficacy to HA/CMC film when applied directly to sites of trauma. In addition, HA/CMC powder reduced adhesions remote from the application site. Importantly, HA/CMC powder did not impair incisional wound healing. On the basis of these results, future investigation of HA/CMC powder is warranted.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Biocompatible Materials / administration & dosage
  • Carboxymethylcellulose Sodium / administration & dosage*
  • Female
  • Hyaluronic Acid / administration & dosage*
  • Laparoscopy / adverse effects
  • Laparoscopy / methods
  • Materials Testing
  • Membranes, Artificial
  • Models, Animal
  • Powders
  • Rabbits
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Adhesions / prevention & control*
  • Wound Healing

Substances

  • Biocompatible Materials
  • Membranes, Artificial
  • Powders
  • Hyaluronic Acid
  • Carboxymethylcellulose Sodium