Crystallization of the Fab from a human monoclonal antibody against gp 41 of human immunodeficiency virus type I

J Mol Biol. 1990 Dec 5;216(3):511-2. doi: 10.1016/0022-2836(90)90377-X.

Abstract

A monoclonal IgG antibody directed against gp 41 from the human immunodeficiency virus (HIV-1) has been crystallized in both intact and Fab forms. Crystals of the intact antibody grow as tetragonal-like prisms too small for conventional X-ray analysis. However, the Fab portion of the antibody produces suitable plate-like crystals which belong to the space group P2(1)2(1)2(1) with unit cell constants of a = 66.5 A, b = 74.3 A and c = 105.3 A. There is one molecule of Fab in the asymmetric unit. The Fab crystals show diffraction to d-spacings less than 3.0 A.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies, Monoclonal / chemistry
  • Antigen-Antibody Reactions
  • Crystallization
  • HIV Envelope Protein gp41 / immunology*
  • HIV-1 / immunology*
  • Immunoglobulin Fab Fragments / chemistry
  • X-Ray Diffraction

Substances

  • Antibodies, Monoclonal
  • HIV Envelope Protein gp41
  • Immunoglobulin Fab Fragments