Attempt to show inhibition by oxygen in the modifying factor assay

Scand J Rheumatol Suppl. 1990:87:81-4. doi: 10.3109/03009749009097062.

Abstract

An Australian laboratory has demonstrated that antibodies to certain strains of enteric bacteria react with cells from HLA-B27-positive patients with a 'modifying factor' (MF) from these bacteria. These observations have proved difficult to reproduce. We investigated the possibility that oxygen inhibits the test by blocking B27, but we were unable to obtain consistent positive results.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / immunology
  • Antigens, Bacterial / analysis*
  • Antigens, Bacterial / immunology
  • Cross Reactions
  • Culture Media
  • Cytotoxicity Tests, Immunologic
  • HLA-B27 Antigen / immunology*
  • Humans
  • Klebsiella / immunology
  • Lymphocytes / immunology
  • Oxidation-Reduction
  • Oxygen / metabolism*
  • Spondylitis, Ankylosing / immunology

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Culture Media
  • HLA-B27 Antigen
  • Klebsiella modifying factor
  • Oxygen