Impact of age and diagnosis on viability during centrifugation and cryopreservation of peripheral blood stem cell products

S Civriz Bozdag; M Bay; E Ayyıldız; P Topcuoglu; O Ilhan

doi:10.1016/j.transci.2012.05.012

Impact of age and diagnosis on viability during centrifugation and cryopreservation of peripheral blood stem cell products

Transfus Apher Sci. 2012 Aug;47(1):117-20. doi: 10.1016/j.transci.2012.05.012. Epub 2012 May 19.

Authors

S Civriz Bozdag¹, M Bay, E Ayyıldız, P Topcuoglu, O Ilhan

Affiliation

¹ Department of Hematology, Ankara University School of Medicine, Ankara, Turkey. [email protected]

PMID: 22609193
DOI: 10.1016/j.transci.2012.05.012

Abstract

Background: The viability of the hematopoietic stem cells infused to the patient is important for transplant outcome.

Study design and methods: We evaluated 31 peripheral blood stem cell product collected from 15 patients. We aimed to check the viabilities of the cells from patients with different age and diagnosis, in different stages of the cryopreservation procedure.

Results: We showed a markedly decreased viability rate after centrifugation and addition of DMSO. Percentages of viabilities were similar between young and old patients in each step. Type of hematological malignancy did not make a significant influence on the viability.

Conclusion: High speed centrifugation has a negative impact on the viability.

MeSH terms

Adult
Age Factors
Aged
Cell Survival
Centrifugation
Cryopreservation*
Female
Hematologic Neoplasms / blood
Hematologic Neoplasms / diagnosis*
Hematologic Neoplasms / therapy*
Hematopoietic Stem Cells*
Humans
Male
Middle Aged
Peripheral Blood Stem Cell Transplantation*
Transplantation, Autologous