Synthesis of adenosine functionalized metal immobilized magnetic nanoparticles for highly selective and sensitive enrichment of phosphopeptides

Chem Commun (Camb). 2012 Jun 25;48(50):6274-6. doi: 10.1039/c2cc31641b. Epub 2012 May 21.

Abstract

A new type of IMAC material, with ATP as the chelating ligand, was synthesized and applied to capture phosphopeptides. For the first time, the approach for phosphopeptide enrichment could provide selectivity under 5000-fold dilution by nonphosphopeptides, and sensitivity of on-target enrichment at 3 amol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / chemistry*
  • Magnetite Nanoparticles / chemistry*
  • Organometallic Compounds / chemical synthesis*
  • Organometallic Compounds / chemistry
  • Particle Size
  • Phosphopeptides / chemistry*
  • Surface Properties

Substances

  • Magnetite Nanoparticles
  • Organometallic Compounds
  • Phosphopeptides
  • Adenosine