A gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion

Yoshitaka Nakayama; Kenjiro Yoshimura; Hidetoshi Iida

doi:10.1128/AEM.01310-12

A gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion

Appl Environ Microbiol. 2012 Aug;78(15):5432-4. doi: 10.1128/AEM.01310-12. Epub 2012 May 18.

Authors

Yoshitaka Nakayama¹, Kenjiro Yoshimura, Hidetoshi Iida

Affiliation

¹ Department of Biology, Tokyo Gakugei University, Koganei-shi, Tokyo, Japan. [email protected]

Abstract

The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acid Transport Systems / genetics*
Amino Acid Transport Systems / metabolism
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Corynebacterium glutamicum / genetics*
Corynebacterium glutamicum / metabolism
Glutamic Acid / metabolism*
Ion Channel Gating / genetics*
Molecular Sequence Data
Mutation, Missense / genetics*
Patch-Clamp Techniques
Species Specificity

Substances

Amino Acid Transport Systems
Bacterial Proteins
Glutamic Acid