Abstract
Peanut/maize intercropping is a sustainable and effective agroecosystem to alleviate iron-deficiency chlorosis. Using suppression subtractive hybridization from the roots of intercropped and monocropped peanut which show different iron nutrition levels, a peanut gene, AhNRAMP1, which belongs to divalent metal transporters of the natural resistance-associated macrophage protein (NRAMP) gene family was isolated. Yeast complementation assays suggested that AhNRAMP1 encodes a functional iron transporter. Moreover, the mRNA level of AhNRAMP1 was obviously induced by iron deficiency in both roots and leaves. Transient expression, laser microdissection, and in situ hybridization analyses revealed that AhNRAMP1 was mainly localized on the plasma membrane of the epidermis of peanut roots. Induced expression of AhNRAMP1 in tobacco conferred enhanced tolerance to iron deprivation. These results suggest that the AhNRAMP1 is possibly involved in iron acquisition in peanut plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arachis / genetics
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Arachis / metabolism*
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Arachis / physiology
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Base Sequence
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Cation Transport Proteins / genetics
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Cation Transport Proteins / metabolism
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Gene Expression Regulation, Plant / physiology*
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Genetic Complementation Test
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Iron / metabolism*
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Iron Deficiencies
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Mutation
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Nicotiana / genetics
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Nicotiana / metabolism
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Nicotiana / physiology
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Phylogeny
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Plant Epidermis / embryology
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Plant Epidermis / genetics
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Plant Epidermis / physiology
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Plant Leaves / genetics
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Plant Leaves / metabolism
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Plant Leaves / physiology
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Plant Roots / genetics
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Plant Roots / metabolism
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Plant Roots / physiology
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Plants, Genetically Modified
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae / physiology
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Seedlings / genetics
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Seedlings / metabolism
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Seedlings / physiology
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Sequence Analysis, DNA
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Zea mays / genetics
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Zea mays / metabolism
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Zea mays / physiology
Substances
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Cation Transport Proteins
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Membrane Proteins
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Plant Proteins
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natural resistance-associated macrophage protein 1
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Iron