Objective: To investigate the association between genetic polymorphism of metabolizing enzymes and DNA repairing enzymes and the susceptibility of pulmonary cancer in Henan population.
Methods: A case-control study of 209 lung cancer patients and 256 healthy control subjects was conducted in Henan population to investigate the role of CYP1A1-Msp1, CYP1A1-exon7, GSTM1, GSTT1, mEH-exon3, mEH-exon4 and XRCC1 in lung cancer. Genotyping was performed by using PCR based restriction fragment length polymorhphism techniques.
Results: The frequency of GSTM1-null, CYP1A1-exon7 mt/mt, mEH-exon3 mt/mt, XRCC1-194 Trp/Trp, XRCC1-280 His/His genotype in the lung cancer case group was significantly higher than that in control group (P < 0.05). The risk of lung cancer was higher in individuals carrying genotypes of GSTM1 (ORadj = 1.76, 95% CI 1.21-2.56, P = 0.005), CYP1A1-exon7 Ile/val + val/val (ORadj = 1.65, 95% CI 1.16-2.39, P = 0.009), mEH-exon3 wt/mt + mt/mt (ORadj = 1.77, 95% CI 1.18-2.64, P = 0.007), XRCC1-194 Arg/Trp + Trp/Trp (ORadj = 1.55, 95% CI 1.07-2.27, P = 0.016) and XRCC1-280 His/His (ORadj = 2.21, 95% CI 1.05-4.52, P = 0.026) than those carrying genotypes of GSTM1 null, CYP1A1-exon7 Ile/Ile, mEH-exon3 wt/wt, XRCC1-194 Arg/Arg and XRCC1-280 Arg/ Arg + Arg/His; There was no significant difference for CYP1A1-Msp1, GSTT1, mEH-exon4, XRCC1-399 genotype between the two groups (P > 0.05).
Conclusion: The genetic polymorphisms of CYP1A1-exon7, GSTM1, mEH-exon3, XRCC1-194 and XRCC1-280 might contribute to the risk of developing lung cancer in Henan population.