Abstract
Chemical investigation of the insect-derived actinomycete Amycolatopsis sp. HCa1 with NMR-guided fractionation led to the isolation of eight new tetrasaccharide derivatives containing an unusual nonreducing glucotetraose skeleton, named actinotetraoses A-H (1-8), and one known compound, tigloside (9). The structures of these compounds were determined on the basis of analyses of their spectroscopic data. The in vitro immunosuppressive activity and cytotoxicity of these compounds were evaluated by T cell viability and MTT assays, respectively. Only actinotetraose E (5) displayed weak immunosuppressive activity.
Georg Thieme Verlag KG Stuttgart · New York.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinobacteria / chemistry*
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Actinobacteria / isolation & purification
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Animals
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Cell Survival / drug effects
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Drug Screening Assays, Antitumor
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Fermentation
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Grasshoppers / microbiology*
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HeLa Cells
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Hep G2 Cells
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Humans
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Immunosuppressive Agents / chemistry
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Immunosuppressive Agents / isolation & purification*
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Immunosuppressive Agents / pharmacology
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Mycelium / chemistry
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Oligosaccharides / chemistry
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Oligosaccharides / isolation & purification*
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Oligosaccharides / pharmacology
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Optical Rotation
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T-Lymphocytes / drug effects*
Substances
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Immunosuppressive Agents
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Oligosaccharides
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actinotetraose E