Development of macrolide lactone antibiotic brefeldin A fermentation process with Eupenicillium brefeldianum ZJB082702

J Biosci Bioeng. 2012 Sep;114(3):262-7. doi: 10.1016/j.jbiosc.2012.04.006. Epub 2012 May 22.

Abstract

In this work, a robust brefeldin A-synthesizing fungus, Eupenicillium brefeldianum ZJB082702, was bred from a Murraya paniculata endophytic fungus E. brefeldianum A1163. Using one-factor-at-a-time experimental design, optimization of media composition for E. brefeldianum ZJB082702 fermenting brefeldin A was conducted. Outcomes indicated that mixed carbon source and mixed nitrogen source were of c ritical importance to brefeldin A fermentation. After 6d culture in the optimized fermentation media, composed of (gl(-1)) 13.33 starch, 26.67 glucose, 1.0 yeast extract powder, 1.0 corn steep liquor, 0.5 soybean meal, 0.75 NaNO(3), 2.5 malt extract, 6.0 CaCO(3), 3.0 MgSO(4), 4.0 KH(2)PO(4), 1.0 × 10(-2) CuSO(4), brefeldin A yield peaked at 1304.7 mgl(-1), 648.2 mgl(-1) in 500 ml baffled flask and 15 l stirred fermentor respectively, formed as a growth associated type of secondary metabolite based on fermentation profile analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / biosynthesis
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / metabolism
  • Bioreactors*
  • Brefeldin A / isolation & purification
  • Brefeldin A / metabolism*
  • Carbon / metabolism
  • Carbon / pharmacology
  • Culture Media / chemistry
  • Culture Media / metabolism
  • Culture Media / pharmacology
  • Eupenicillium / drug effects
  • Eupenicillium / growth & development
  • Eupenicillium / metabolism*
  • Fermentation / drug effects
  • Nitrogen / metabolism
  • Nitrogen / pharmacology

Substances

  • Anti-Bacterial Agents
  • Culture Media
  • Brefeldin A
  • Carbon
  • Nitrogen