Abstract
The potential of lipopolysaccharide (LPS) to induce antigen-specific B cell responses to acetylcholine receptor (AChR) in myasthenia gravis (MG) was evaluated in wild type (WT) and CD4-/- C57BL/6 mice. The WT mice immunized with AChR in LPS developed an MG-like disease (LPS-EAMG) similar to that induced by immunization with AChR in complete Freund's adjuvant (CFA-EAMG). CD4-/- mice were resistant to CFA-EAMG but susceptible to LPS-EAMG. LPS abrogated EAMG resistance in CD4-/- mice by increasing high-affinity anti-AChR IgG2b in sera and enhancing immune complex deposition in muscle.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / pharmacology*
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Animals
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Antigen-Antibody Complex / immunology
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Autoantibodies / blood
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Autoantibodies / immunology
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Autoantigens / immunology
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CD4-Positive T-Lymphocytes / immunology*
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Enzyme-Linked Immunosorbent Assay
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Freund's Adjuvant / immunology
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Freund's Adjuvant / pharmacology
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Lipopolysaccharides / immunology
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Lipopolysaccharides / pharmacology*
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Lymphocyte Activation / immunology*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Microscopy, Fluorescence
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Myasthenia Gravis, Autoimmune, Experimental / immunology*
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Receptors, Cholinergic / immunology
Substances
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Adjuvants, Immunologic
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Antigen-Antibody Complex
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Autoantibodies
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Autoantigens
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Lipopolysaccharides
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Receptors, Cholinergic
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Freund's Adjuvant