Abstract
We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
Copyright © 2012 Elsevier B.V. All rights reserved.
MeSH terms
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Animals
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Bacterial Vaccines / genetics*
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Bacterial Vaccines / immunology
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Bacterial Vaccines / isolation & purification
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Cattle
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Cattle Diseases / immunology
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Cattle Diseases / microbiology*
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Cattle Diseases / prevention & control
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Chlamydia / genetics*
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Chlamydia / immunology
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Chlamydia / isolation & purification
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Chlamydia Infections / immunology
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Chlamydia Infections / microbiology
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Chlamydia Infections / prevention & control
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Chlamydia Infections / veterinary*
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DNA, Bacterial / genetics
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Goat Diseases / immunology
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Goat Diseases / microbiology*
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Goat Diseases / prevention & control
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Goats
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Molecular Typing
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Polymerase Chain Reaction
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Polymorphism, Single Nucleotide
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Sheep
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Sheep Diseases / immunology
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Sheep Diseases / microbiology*
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Sheep Diseases / prevention & control
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Transition Temperature
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Vaccines, Live, Unattenuated / genetics
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Vaccines, Live, Unattenuated / immunology
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Vaccines, Live, Unattenuated / isolation & purification
Substances
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Bacterial Vaccines
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DNA, Bacterial
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Vaccines, Live, Unattenuated