Western blot analysis as an aid for the diagnosis of cutaneous leishmaniasis due to Leishmania major

Christelle Pomares; Laura Despierres; Pascal del Giudice; Pascal Delaunay; Grégory Michel; Bernard Ferrua; Pierre Marty

doi:10.1016/j.trstmh.2012.03.001

Western blot analysis as an aid for the diagnosis of cutaneous leishmaniasis due to Leishmania major

Trans R Soc Trop Med Hyg. 2012 Jul;106(7):452-4. doi: 10.1016/j.trstmh.2012.03.001. Epub 2012 May 30.

Authors

Christelle Pomares¹, Laura Despierres, Pascal del Giudice, Pascal Delaunay, Grégory Michel, Bernard Ferrua, Pierre Marty

Affiliation

¹ Faculté de Médecine, Université de Nice-Sophia Antipolis, Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Nice, Nice, France.

PMID: 22657532
DOI: 10.1016/j.trstmh.2012.03.001

Abstract

Cutaneous leishmaniasis (CL) due to Leishmania major is endemic in the Old World. To evaluate the diagnostic value of Western blot (WB) compared with IFAT, we tested serum samples from 45 patients with proven CL. Twenty-one (47%) patients were positive by IFAT and all patients were positive by WB with specific bands against 14kDa and/or 18kDa Leishmania antigens. Our results suggest that WB could be a useful non-invasive tool for the diagnosis of CL caused by L. major.

MeSH terms

Animals
Antibodies, Protozoan / immunology
Blotting, Western*
Female
Humans
Leishmania infantum / immunology
Leishmania infantum / isolation & purification*
Leishmania major / immunology
Leishmania major / isolation & purification*
Leishmaniasis, Cutaneous / immunology
Leishmaniasis, Cutaneous / pathology*
Male
Polymerase Chain Reaction
Sensitivity and Specificity
Skin / parasitology
Skin / pathology*

Substances

Antibodies, Protozoan