Vitamin K1-loaded lipid-core nanocapsules: physicochemical characterization and in vitro skin permeation

Skin Res Technol. 2013 Feb;19(1):e223-30. doi: 10.1111/j.1600-0846.2012.00631.x. Epub 2012 Jun 7.

Abstract

Background: The incorporation of substances in nanocarriers can modulate and/or manage their delivery profiles (immediate or sustained) and permeation through skin. Consequently, drug nanencapsulation intended for topical treatment can reduce the systemic absorption of the substance.

Objective: To obtain and characterize vitamin K1-loaded lipid core nanocapsules as well as to determine whether the nanoencapsulation influences the skin permeation of this vitamin.

Methods: The skin permeation study was performed by means of Franz-type diffusion cells followed by the tape stripping and retention techniques. The vitamin K1-loaded lipid core nanocapsules were obtained by the preformed polymer precipitation method and the particles were characterized.

Results: The nanocapsules presented average diameter of 211 ± 2 nm, pH of 5.7 ± 0.3, zeta potential of -14.9 ± 0.6 mV and drug content of 10.2 mg/mL (102.1%). The physical stability of the nanocapsule suspension was verified using multiple light backscattering analysis. The amount of vitamin K1 in the dermis after 8 h of drug permeation was higher when the nanocapsules were applied compared to the control. Moreover, retention in the outermost skin layer and a decrease in the skin permeation to the receptor compartment due to the nanoencapsulation were observed.

Conclusion: Thus, nanoencapsulation can lead to the selective permeation of vitamin K1 through the skin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drug Carriers / chemistry
  • Drug Carriers / pharmacokinetics*
  • Female
  • In Vitro Techniques
  • Lipids / chemistry
  • Nanocapsules / chemistry*
  • Particle Size
  • Polymers / chemistry
  • Polymers / pharmacokinetics
  • Skin / metabolism*
  • Skin Absorption
  • Swine
  • Viscosity
  • Vitamin K 1 / pharmacokinetics*
  • Vitamins / pharmacokinetics*

Substances

  • Drug Carriers
  • Lipids
  • Nanocapsules
  • Polymers
  • Vitamins
  • Vitamin K 1