Abstract
Adult rats were treated acutely with peripheral kainic acid (KA), and changes in brain-derived neurotrophic factor (BDNF) mRNA and protein were tracked over time across multiple brain regions. Despite robust elevation in both mRNA and protein in multiple brain regions, plasma BDNF was unchanged and cerebrospinal fluid (CSF) BDNF levels remained undetectable. Primary neurons were then treated with KA. BDNF was similarly elevated within neurons, but was undetectable in neuronal media. Thus, while deficits in BDNF signaling have been implicated in a number of diseases, these data suggest that extracellular concentrations of BDNF may not be a facile biomarker for changes in neurons.
MeSH terms
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Animals
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Brain / metabolism
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Brain / pathology
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Brain-Derived Neurotrophic Factor / blood*
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Brain-Derived Neurotrophic Factor / cerebrospinal fluid
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Brain-Derived Neurotrophic Factor / genetics*
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Brain-Derived Neurotrophic Factor / metabolism
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Cells, Cultured
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Choroid Plexus / pathology
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Gene Expression*
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Hippocampus / metabolism*
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Hippocampus / pathology
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Humans
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Kainic Acid
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L-Lactate Dehydrogenase / metabolism
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Male
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Neurons / enzymology
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Neurons / metabolism
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Primary Cell Culture
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Seizures / blood
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Seizures / cerebrospinal fluid
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Seizures / chemically induced
Substances
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Brain-Derived Neurotrophic Factor
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RNA, Messenger
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L-Lactate Dehydrogenase
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Kainic Acid