Abstract
Experimental data are presented which were obtained under comparative evaluation of influence of different freezing-thawing conditions on antioxidant properties of isolated proteins: human serum albumin, cytochrome c from the horse heart and glucose oxidase from Aspergillus niger. The observed protein antioxidant activity alterations are assumed to be a result of protein conformational changes. The character of freezing-thawing influence on the protein antioxidant activity depends on the molecular structure and cooling conditions.
MeSH terms
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Animals
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Antioxidants / chemistry*
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Aspergillus niger
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Benzothiazoles / chemistry
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Cryopreservation
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Cytochromes c / chemistry*
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Ferrous Compounds / chemistry
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Ferrozine / chemistry
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Freezing
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Glucose Oxidase / chemistry*
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Horses
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Humans
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Protein Conformation
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Serum Albumin / chemistry*
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Solutions
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Spectrophotometry
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Sulfonic Acids / chemistry
Substances
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Antioxidants
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Benzothiazoles
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Ferrous Compounds
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Serum Albumin
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Solutions
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Sulfonic Acids
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Ferrozine
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2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
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Cytochromes c
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Glucose Oxidase
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ferrous chloride