Protective effects of 20(s)-protopanaxtriol on viral myocarditis infected by coxsackievirus B3

Pathobiology. 2012;79(6):285-9. doi: 10.1159/000331229. Epub 2012 Jun 9.

Abstract

Objective: Coxsackievirus B3 (CVB3) is a dominant causative agent for viral myocarditis. So far, effective therapies for the treatment of the disease are not available. 20(S)-Protopanaxtriol is a major component of Panax pseudoginseng and has been clinically used for the treatment of heart diseases. However, it is not known whether 20(S)-protopanaxtriol exerts any anti-viral effects. Thus, the aim of this study was to investigate the therapeutic effects of 20(S)-protopanaxtriol against CVB3 in vivo and in vitro.

Methods: The antiviral effects of 20(S)-protopanaxtriol in vitro were evaluated in HeLa cells infected by CVB3. Then, we examined the protective effects of 20(S)-protopanaxtriol on CVB3-induced myocarditis in BALB/c mice. These mice were treated with 20(S)-protopanaxtriol at doses of 100-400 mg·kg(-1)·day(-1) for 7 days and compared with the controls.

Results: We found that 20(S)-protopanaxtriol possessed potent antiviral effects on CVB3 in vitro. Compared with control mice, virus titers and pathological changes in the hearts were significantly decreased in the 20(S)-protopanaxtriol-treated group. Furthermore, biochemical markers of myocardial injury such as plasma lactate dehydrogenase and creatine kinase were decreased to normal levels.

Conclusions: These data provide the possibility that 20(S)-protopanaxtriol can be used as a potential therapeutic means for treatment of viral myocarditis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Antiviral Agents / therapeutic use
  • Cell Survival
  • Coxsackievirus Infections / drug therapy*
  • Coxsackievirus Infections / pathology
  • Coxsackievirus Infections / virology
  • Creatine Kinase / blood
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical
  • Enterovirus B, Human / drug effects*
  • HeLa Cells
  • Humans
  • L-Lactate Dehydrogenase / blood
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Myocarditis / drug therapy*
  • Myocarditis / pathology
  • Myocarditis / virology
  • Panax / chemistry*
  • Plants, Medicinal / chemistry*
  • Triterpenes / chemistry
  • Triterpenes / pharmacology
  • Triterpenes / therapeutic use*
  • Viral Load

Substances

  • Antiviral Agents
  • Triterpenes
  • protopanaxtriol
  • L-Lactate Dehydrogenase
  • Creatine Kinase