Site-specific incorporation of ε-N-crotonyllysine into histones

Angew Chem Int Ed Engl. 2012 Jul 16;51(29):7246-9. doi: 10.1002/anie.201203349. Epub 2012 Jun 11.

Abstract

A novel post-translationally modified amino acid, crotonyllysine (Kcr), was genetically incorporated into proteins in bacterial and mammalian cells using an evolved pyrrolysyl-tRNA/synthetase-tRNA pair. The ability to produce histones with homogenous, site-specific Kcr modifications will be valuable in elucidating the biological role of this recently identified post-translational modification.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acyl-tRNA Synthetases / genetics
  • Amino Acyl-tRNA Synthetases / metabolism
  • Cell Line
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Histones / chemistry
  • Histones / genetics
  • Histones / metabolism*
  • Humans
  • Lysine / analogs & derivatives*
  • Lysine / chemistry
  • Lysine / genetics
  • Lysine / metabolism
  • Mutation
  • Protein Engineering*
  • Protein Processing, Post-Translational*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism

Substances

  • Histones
  • Recombinant Proteins
  • epsilon-N-crotonyllysine
  • Amino Acyl-tRNA Synthetases
  • Lysine