Cyclic caged morpholinos: conformationally gated probes of embryonic gene function

Angew Chem Int Ed Engl. 2012 Jul 9;51(28):6908-11. doi: 10.1002/anie.201201690. Epub 2012 Jun 11.

Abstract

Feeling a bit cagey: morpholino-based antisense reagents have been caged through oligonucleotide cyclization, enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Differentiation
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / metabolism*
  • Gene Expression Regulation, Developmental*
  • Gene Expression*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Larva / cytology
  • Larva / metabolism*
  • Morpholinos / pharmacology*
  • Oligonucleotides / pharmacology*
  • Organogenesis
  • Pancreas / embryology
  • Pancreas / metabolism*
  • Zebrafish / embryology
  • Zebrafish / genetics
  • Zebrafish / metabolism

Substances

  • Morpholinos
  • Oligonucleotides
  • Green Fluorescent Proteins