GDNF released from microspheres enhances nerve regeneration after delayed repair

Muscle Nerve. 2012 Jul;46(1):122-4. doi: 10.1002/mus.23295.

Abstract

Introduction: Delays in surgical repair following nerve transection produce progressively inferior motor nerve regeneration. Regeneration can be improved with delivery of exogenous growth factor. We developed a delivery system that could be applied at the nerve repair site to deliver growth factors locally to regenerating nerve.

Methods: Poly(lactic-co-glycolic acid) microspheres containing glial-derived neurotrophic factor (GDNF) suspended within fibrin were developed into a delivery system for local application surrounding nerve at a repair site in an experimental rat model.

Results: The system containing GDNF remained at the injury site for up to 2 weeks and improved motor nerve regeneration following chronic axotomy and denervation.

Conclusions: Based on the positive outcome of the delivery system, we plan to study the delivery system over longer time courses of release and nerve regeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / drug effects*
  • Axons / physiology
  • Axotomy
  • Denervation
  • Drug Delivery Systems
  • Glial Cell Line-Derived Neurotrophic Factor / pharmacology*
  • Microspheres
  • Nerve Regeneration / drug effects*
  • Nerve Regeneration / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Wound Healing / drug effects

Substances

  • Glial Cell Line-Derived Neurotrophic Factor