A facile method to synthesize histones with posttranslational modification mimics

Biochemistry. 2012 Jul 3;51(26):5232-4. doi: 10.1021/bi300535a. Epub 2012 Jun 19.

Abstract

Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetylation
  • Histones / chemical synthesis*
  • Histones / chemistry*
  • Immunoprecipitation
  • Lysine / chemistry
  • Methylation
  • Molecular Structure
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Serine / chemistry

Substances

  • Histones
  • Serine
  • Lysine