Diagnosis of human parechovirus infections of the central nervous system with a commercial real-time reverse transcription-polymerase chain reaction kit and direct genotyping of cerebrospinal fluid specimens

Audrey Mirand; Christine Archimbaud; Martine Chambon; Christel Regagnon; Amélie Brebion; Jean-Luc Bailly; Hélène Peigue-Lafeuille; Cécile Henquell

doi:10.1016/j.diagmicrobio.2012.05.017

Diagnosis of human parechovirus infections of the central nervous system with a commercial real-time reverse transcription-polymerase chain reaction kit and direct genotyping of cerebrospinal fluid specimens

Diagn Microbiol Infect Dis. 2012 Sep;74(1):78-80. doi: 10.1016/j.diagmicrobio.2012.05.017. Epub 2012 Jun 12.

Authors

Audrey Mirand¹, Christine Archimbaud, Martine Chambon, Christel Regagnon, Amélie Brebion, Jean-Luc Bailly, Hélène Peigue-Lafeuille, Cécile Henquell

Affiliation

¹ CHU Clermont-Ferrand, Laboratoire de Virologie, Centre de Biologie, F-63003 Clermont-Ferrand, France. [email protected]

PMID: 22698801
DOI: 10.1016/j.diagmicrobio.2012.05.017

Abstract

We screened 100 cerebrospinal fluid specimens for the human parechoviruses (HPeV) genome with the commercial parechovirus r-gene™ kit, which allows results to be available in a clinically relevant time frame. HPeV infection was diagnosed in 4 infants (<4 months) and all genotyped viruses were HPeV3.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Cerebrospinal Fluid / virology*
Child
Child, Preschool
Encephalitis, Viral / diagnosis*
Encephalitis, Viral / virology
Female
Genotype
Humans
Infant
Infant, Newborn
Male
Middle Aged
Parechovirus / classification
Parechovirus / genetics
Parechovirus / isolation & purification*
Picornaviridae Infections / diagnosis*
Picornaviridae Infections / virology
Real-Time Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Virology / methods
Young Adult