Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains

Acta Microbiol Hung. 1990;37(2):201-6.

Abstract

The validity of CAMP tests with Staphylococcus aureus and Rhodococcus (Corynebacterium) equi as defined for Listeria identification was revised. This characterization method appeared to be unreliable for two reasons: first, a positive CAMP test with R. equi is not specific for Listeria ivanovii as Listeria monocytogenes (and Listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with S. aureus when assaying haemolytic and non-haemolytic Listeria strains (possibility of false negative and false positive results; subjectivity of the interpretation). The use of a Microplate technique previously described instead of CAMP tests is proposed for the reliable demonstration of the haemolytic character of Listeria in the routine identification of these organisms.

MeSH terms

  • Bacterial Typing Techniques*
  • Diagnostic Errors
  • Evaluation Studies as Topic
  • Food Microbiology
  • Hemolysis*
  • Listeria / classification*
  • Rhodococcus
  • Staphylococcus aureus