Abstract
The 90 % human cytomegalovirus inhibitory concentration of 17-allylamino-17-(demethoxy)geldanamycin (17-AAG) was 0.1 nM and 50 % cytotoxicity required at least a 10 μM concentration. Three molecular targets may explain the antiviral activities of this compound. These are (1) heat shock protein maturation complexes, (2) host cell cycle progression and (3) phosphatidylinositol 3-kinase signaling. However, the data suggested a mechanism of action where 17-AAG blocked immediate-early protein transactivation.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Benzoquinones / pharmacology*
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Cells, Cultured
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Cytomegalovirus / drug effects*
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Cytomegalovirus / metabolism
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Cytomegalovirus / physiology
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Fibroblasts / virology
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Humans
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Immediate-Early Proteins / antagonists & inhibitors*
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Immediate-Early Proteins / metabolism
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Lactams, Macrocyclic / pharmacology*
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Transcriptional Activation / drug effects
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Virus Replication / drug effects*
Substances
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Benzoquinones
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Immediate-Early Proteins
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Lactams, Macrocyclic
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tanespimycin