Over-expression of rice leucine-rich repeat protein results in activation of defense response, thereby enhancing resistance to bacterial soft rot in Chinese cabbage

Plant Cell Rep. 2012 Oct;31(10):1845-50. doi: 10.1007/s00299-012-1298-9. Epub 2012 Jun 21.

Abstract

Pectobacterium carotovorum subsp. carotovorum causes soft rot disease in various plants, including Chinese cabbage. The simple extracellular leucine-rich repeat (eLRR) domain proteins have been implicated in disease resistance. Rice leucine-rich repeat protein (OsLRP), a rice simple eLRR domain protein, is induced by pathogens, phytohormones, and salt. To see whether OsLRP enhances disease resistance to bacterial soft rot, OsLRP was introduced into Chinese cabbage by Agrobacterium-mediated transformation. Two independent transgenic lines over-expressing OsLRP were generated and further analyzed. Transgenic lines over-expressing OsLRP showed enhanced disease resistance to bacterial soft rot compared to non-transgenic control. Bacterial growth was retarded in transgenic lines over-expressing OsLRP compared to non-transgenic controls. We propose that OsLRP confers enhanced resistance to bacterial soft rot. Monitoring expression of defense-associated genes in transgenic lines over-expressing OsLRP, two different glucanases and Brassica rapa polygalacturonase inhibiting protein 2, PDF1 were constitutively activated in transgenic lines compared to non-transgenic control. Taken together, heterologous expression of OsLRP results in the activation of defense response and enhanced resistance to bacterial soft rot.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium / genetics
  • Agrobacterium / metabolism
  • Brassica rapa / genetics
  • Brassica rapa / immunology
  • Brassica rapa / microbiology*
  • Disease Resistance*
  • Gene Expression Regulation, Plant
  • Leucine-Rich Repeat Proteins
  • Oryza / genetics*
  • Pectobacterium carotovorum / pathogenicity*
  • Plant Diseases / immunology*
  • Plant Diseases / microbiology
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / immunology
  • Plants, Genetically Modified / microbiology
  • Proteins / genetics
  • Proteins / immunology
  • Proteins / metabolism*
  • Stress, Physiological
  • Transformation, Genetic
  • Transgenes

Substances

  • Leucine-Rich Repeat Proteins
  • Plant Proteins
  • Proteins