Abstract
We present the evolution of testing algorithms at our institution in which the C. Diff Quik Chek Complete immunochromatographic cartridge assay determines the presence of both glutamate dehydrogenase and Clostridium difficile toxins A and B as a primary screen for C. difficile infection and indeterminate results (glutamate dehydrogenase positive, toxin A and B negative) are confirmed by the GeneXpert C. difficile PCR assay. This two-step algorithm is a cost-effective method for highly sensitive detection of toxigenic C. difficile.
MeSH terms
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Algorithms
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology
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Bacterial Toxins / genetics
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Bacterial Toxins / immunology
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Bacteriological Techniques / methods*
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Clostridioides difficile / isolation & purification*
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Clostridium Infections / diagnosis*
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Clostridium Infections / microbiology*
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Enterotoxins / genetics
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Enterotoxins / immunology
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Glutamate Dehydrogenase / genetics
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Glutamate Dehydrogenase / immunology
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Hospitals, University
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Humans
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Immunoassay / methods
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Polymerase Chain Reaction / methods
Substances
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Bacterial Proteins
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Bacterial Toxins
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Enterotoxins
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tcdA protein, Clostridium difficile
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toxB protein, Clostridium difficile
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Glutamate Dehydrogenase