Simple and reproducible hepatectomy in the mouse using the clip technique

World J Gastroenterol. 2012 Jun 14;18(22):2767-74. doi: 10.3748/wjg.v18.i22.2767.

Abstract

Aim: To investigate the reliability of massive hepatectomy models by using clip techniques.

Methods: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.

Results: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques.

Conclusion: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.

Keywords: Animal model; Clip; Hepatectomy; Microsurgery; Surgical technique.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Hepatectomy / instrumentation*
  • Hepatectomy / methods*
  • Learning Curve
  • Liver / pathology
  • Liver / surgery*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Models, Animal
  • Necrosis
  • Organ Size
  • Reproducibility of Results
  • Surgical Instruments*
  • Suture Techniques
  • Time Factors