Mitogen activated protein kinases blockade improves lipopolysaccharide-induced ileal motor disturbances

Rev Esp Enferm Dig. 2012 Jun;104(6):305-9. doi: 10.4321/s1130-01082012000600004.
[Article in English, Spanish]

Abstract

Background: several diseases such as sepsis can affect the ileum. Lipopolysaccharide (LPS), an endotoxin present in the cell wall of gram negative bacteria, is a causative agent of sepsis.

Objectives: the aims of this study were: a) to investigate the role of mitogen activated protein kinases (MAPKs) in the effect of LPS on the acetylcholine-induced contractions of rabbit ileum; and b) to study the localization of MAPKs in the ileum.

Material and methods: ileal contractility was studied in an organ bath and MAPKs were localized by immunohistochemistry.

Results: acetylcholine-induced contractions decreased with LPS. SB203580, SP600125 and U0126 blocked the effect of LPS on the acetylcholine-induced contractions. Phosphorylated p38 and ERK were detected in neurons of myenteric plexus and Phosphorylated p38 and JNK in smooth muscle cells of ileum.

Conclusion: we can suggest that p38, JNK, and ERK MAPKs are involved in the mechanism of action of LPS in the ileum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / physiology
  • Animals
  • Anthracenes / pharmacology
  • Biomarkers / metabolism
  • Butadienes / pharmacology
  • Enzyme Inhibitors / pharmacology*
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Ileum / drug effects
  • Ileum / metabolism
  • Ileum / microbiology
  • Ileum / physiopathology*
  • Imidazoles / pharmacology
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Lipopolysaccharides / adverse effects*
  • Male
  • Mitogen-Activated Protein Kinases / metabolism*
  • Muscle Contraction / drug effects
  • Muscle Contraction / physiology*
  • Nitriles / pharmacology
  • Pyridines / pharmacology
  • Rabbits
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Anthracenes
  • Biomarkers
  • Butadienes
  • Enzyme Inhibitors
  • Imidazoles
  • Lipopolysaccharides
  • Nitriles
  • Pyridines
  • U 0126
  • lipopolysaccharide, Escherichia coli O111 B4
  • pyrazolanthrone
  • Extracellular Signal-Regulated MAP Kinases
  • JNK Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases
  • Acetylcholine
  • SB 203580